Selective plane illumination microscopy on a chip.

ABSTRACT

Selective plane illumination microscopy can image biological samples at a high spatiotemporal resolution. Complex sample preparation and system alignment normally limit the throughput of the method. Using femtosecond laser micromachining, we created an integrated optofluidic device that allows obtaining continuous flow imaging, three-dimensional reconstruction and high-throughput analysis of large multicellular spheroids at a subcellular resolution.