Alternative Computational Analysis Shows No Evidence for Nucleosome Enrichment at Repetitive Sequences in Mammalian Spermatozoa.
Dev Cell
; 37(1): 98-104, 2016 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-27046835
ABSTRACT
Samans et al. (2014) reported the enrichment of nucleosomes in human and bovine spermatozoa at centromere repeats and retrotransposon sequences such as LINE-1 and SINE. We demonstrate here that nucleosomal enrichments at repetitive sequences as reported result from bioinformatic analyses that make redundant use of sequencing reads that map to multiple locations in the genome. To illustrate that this computational approach is flawed, we observed comparable artificial enrichments at repetitive sequences when aligning control genomic DNA or simulated reads of uniform genome coverage. These results imply that the main conclusions of the article by Samans et al. (2014) are confounded by an inappropriate computational methodology used to analyze the primary data.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
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DNA
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Nucleossomos
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Sequências Repetitivas de Ácido Nucleico
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Regulação da Expressão Gênica
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Regiões Promotoras Genéticas
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Montagem e Desmontagem da Cromatina
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Dev Cell
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
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