Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. coli.

Malik, Ajamaluddin; Alsenaidy, Abdulrahman M; Elrobh, Mohamed; Khan, Wajahatullah; Alanazi, Mohammed S; Bazzi, Mohammad D

Malik, Ajamaluddin; Alsenaidy, Abdulrahman M; Elrobh, Mohamed; Khan, Wajahatullah; Alanazi, Mohammed S; Bazzi, Mohammad D.

Afiliação

Malik A; Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
Alsenaidy AM; Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
Elrobh M; Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia; Biochemistry Department, Faculty of Science, Ain-Shams University, Abbassia 11381, Cairo, Egypt.
Khan W; Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, PO Box 3660, Riyadh 11426, Saudi Arabia.
Alanazi MS; Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
Bazzi MD; Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia; Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.

Saudi J Biol Sci ; 23(3): 410-9, 2016 May.

Article em En | MEDLINE | ID: mdl-27081368

Palavras-chave

2× LB, double strength LuriaBertani; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; Expression optimization; FPLC, fast protein liquid chromatography; Fast protein liquid chromatography; Heat shock protein; Hsp70; IPTG, isopropyl ß-d-1-thiogalactopyranoside; LB, LuriaBertani; Molecular chaperone; NB, nutrient broth; NiNTA, nickelnitrilotriacetic acid; OD600, optical density at 600 nm; PMSF, phenylmethylsulfonyl fluoride; Recombinant; TB, terrific broth; amp, ampicillin; rpm, rotations per minute

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Saudi J Biol Sci Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Arábia Saudita

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Saudi J Biol Sci Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Arábia Saudita