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Developing a fluorescence-coupled capillary electrophoresis based method to probe interactions between QDs and colorectal cancer targeting peptides.
Liu, Feifei; Wang, Jianhao; Yang, Li; Liu, Li; Ding, Shumin; Fu, Minli; Deng, Linhong; Gao, Li-Qian.
Afiliação
  • Liu F; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Wang J; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Yang L; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Liu L; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Ding S; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Fu M; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Deng L; School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
  • Gao LQ; Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu, P. R. China.
Electrophoresis ; 37(15-16): 2170-4, 2016 08.
Article em En | MEDLINE | ID: mdl-27159348
As is well known, quantum dots (QDs) have become valuable probes for cancer imaging. In particular, QD-labeled targeting peptides are capable of identifying cancer or tumors cells. A new colorectal cancer targeting peptide, cyclo(1, 9)-CTPSPFSHC, has strong targeting ability and also shows great potential in the identification and treatment of colon cancer. Herein, we synthesized a dual functional polypeptide, cyclo(1, 9)-CTPSPFSHCD2 G2 DP9 G3 H6 (H6 -TCP), to investigate its interaction with QDs inside the capillary. Fluorescence-coupled CE was adopted and applied to characterize the self-assembly of H6 -TCP onto QDs. It was indicated that the formation of the assembly was affected by H6 -TCP/QD molar ratio and sampling time. This novel in-capillary assay greatly reduced the sample consumption and the detection time, which was beneficial for the environment. It is expected that this kind of detection method could find more applications to provide more useful information for cancer diagnosis and detection of harm and hazardous substances/organisms in the environment in the future.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Colorretais / Sondas Moleculares / Eletroforese Capilar Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Electrophoresis Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Colorretais / Sondas Moleculares / Eletroforese Capilar Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Electrophoresis Ano de publicação: 2016 Tipo de documento: Article