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Demineralized dentin matrix combined with recombinant human bone morphogenetic protein-2 in rabbit calvarial defects.
Um, In-Woong; Hwang, Suk-Hyun; Kim, Young-Kyun; Kim, Moon-Young; Jun, Sang-Ho; Ryu, Jae-Jun; Jang, Hyon-Seok.
Afiliação
  • Um IW; R&D Institute, Korea Tooth Bank, Seoul, Korea.
  • Hwang SH; Department of Medicine, Korea University Graduate School, Seoul, Korea.
  • Kim YK; Department of Oral and Maxillofacial Surgery, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.
  • Kim MY; Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, Cheonan, Korea.
  • Jun SH; Department of Dentistry, Korea University Anam Hospital, Seoul, Korea.
  • Ryu JJ; Department of Dentistry, Korea University Anam Hospital, Seoul, Korea.
  • Jang HS; Department of Dentistry, Korea University Ansan Hospital, Ansan, Korea.
J Korean Assoc Oral Maxillofac Surg ; 42(2): 90-8, 2016 Apr.
Article em En | MEDLINE | ID: mdl-27162749
OBJECTIVES: The aim of this study was to compare the osteogenic effects of demineralized dentin matrix (DDM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in rabbit calvarial defects with DDM and anorganic bovine bone (ABB) combined with rhBMP-2. MATERIALS AND METHODS: Four round defects with 8-mm diameters were created in each rabbit calvaria. Each defect was treated with one of the following: 1) DDM, 2) ABB/rhBMP-2, or 3) DDM/rhBMP-2. The rhBMP-2 was combined with DDM and ABB according to a stepwise dry and dip lyophilizing protocol. Histological and microcomputed tomography (µCT) analyses were performed to measure the amount of bone formation and bone volume after 2- and 8-week healing intervals. RESULTS: Upon histological observation at two weeks, the DDM and ABB/rhBMP-2 groups showed osteoconductive bone formation, while the DDM/rhBMP-2 group showed osteoconductive and osteoinductive bone formation. New bone formation was higher in DDM/rhBMP-2, DDM and ABB decreasing order. The amounts of bone formation were very similar at two weeks; however, at eight weeks, the DDM/rhBMP-2 group showed a two-fold greater amount of bone formation compared to the DDM and ABB/rhBMP-2 groups. The µCT analysis showed markedly increased bone volume in the DDM/rhBMP-2 group at eight weeks compared with that of the DDM group. Notably, there was a slight decrease in bone volume in the ABB/rhBMP-2 group at eight weeks. There were no significant differences among the DDM, ABB/rhBMP-2, and DDM/rhBMP-2 groups at two or eight weeks. CONCLUSION: Within the limitations of this study, DDM appears to be a suitable carrier for rhBMP-2 in orthotopic sites.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: J Korean Assoc Oral Maxillofac Surg Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: J Korean Assoc Oral Maxillofac Surg Ano de publicação: 2016 Tipo de documento: Article