Translin and Trax differentially regulate telomere-associated transcript homeostasis.
Oncotarget
; 7(23): 33809-20, 2016 Jun 07.
Article
em En
| MEDLINE
| ID: mdl-27183912
ABSTRACT
Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
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RNA Fúngico
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RNA Mensageiro
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Proteínas de Transporte
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Telômero
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Proteínas de Ligação a RNA
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Proteínas de Schizosaccharomyces pombe
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Proteínas de Ligação a DNA
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Homeostase do Telômero
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RNA Longo não Codificante
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Oncotarget
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Reino Unido