Overcoming the Refractory Expression of Secreted Recombinant Proteins in Mammalian Cells through Modification of the Signal Peptide and Adjacent Amino Acids.

Güler-Gane, Gülin; Kidd, Sara; Sridharan, Sudharsan; Vaughan, Tristan J; Wilkinson, Trevor C I; Tigue, Natalie J

Güler-Gane, Gülin; Kidd, Sara; Sridharan, Sudharsan; Vaughan, Tristan J; Wilkinson, Trevor C I; Tigue, Natalie J.

Afiliação

Güler-Gane G; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.
Kidd S; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.
Sridharan S; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.
Vaughan TJ; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.
Wilkinson TC; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.
Tigue NJ; Department of Antibody Discovery and Protein Engineering, MedImmune Ltd., Granta Park, Cambridge, United Kingdom.

PLoS One ; 11(5): e0155340, 2016.

Article em En | MEDLINE | ID: mdl-27195765

Assuntos

Aminoácidos/metabolismo; Sinais Direcionadores de Proteínas; Proteínas Recombinantes/metabolismo; Fosfatase Alcalina/metabolismo; Transporte Biológico; Meios de Cultivo Condicionados/química; Vetores Genéticos; Células HEK293; Humanos; Interferon-alfa/metabolismo; Processamento de Proteína Pós-Traducional

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Sinais Direcionadores de Proteínas / Aminoácidos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Reino Unido

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