Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA.
Acta Virol
; 60(2): 151-5, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27265464
ABSTRACT
As for the alternative AUGs in foot-and-mouth disease virus (FMDV), nucleotide bias of the context flanking the AUG(2nd) could be used as a strong signal to initiate translation. To determine the role of the specific nucleotide context, dicistronic reporter constructs were engineered to contain different versions of nucleotide context linking between internal ribosome entry site (IRES) and downstream gene. The results indicate that under FMDV IRES-dependent mechanism, the nucleotide contexts flanking start codon can influence the translation initiation efficiencies. The most optimal sequences for both start codons have proved to be UUU AUG(1st) AAC and AAG AUG(2nd) GAA.
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Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Códon de Iniciação
/
Vírus da Febre Aftosa
/
Febre Aftosa
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Virol
Ano de publicação:
2016
Tipo de documento:
Article