Kit receptor tyrosine kinase dysregulations in feline splenic mast cell tumours.
Vet Comp Oncol
; 15(3): 1051-1061, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-27278268
ABSTRACT
This study investigated Kit receptor dysregulations (cytoplasmic immunohistochemical expression and/or c-KIT mutations) in cats affected with splenic mast cell tumours. Twenty-two cats were included. Median survival time was 780 days (range 1-1219). An exclusive splenic involvement was significantly (P = 0.042) associated with longer survival (807 versus 120 days). Eighteen tumours (85.7%) showed Kit cytoplasmic expression (Kit pattern 2, 3). Mutation analysis was successful in 20 cases. Fourteen missense mutations were detected in 13 out of 20 tumours (65%). Eleven (78.6%) were located in exon 8, and three (21.6%) in exon 9. No mutations were detected in exons 11 and 17. Seven mutations corresponded to the same internal tandem duplication in exon 8 (c.1245_1256dup). Although the association between Kit cytoplasmic expression and mutations was significant, immunohistochemistry cannot be considered a surrogate marker for mutation analysis. No correlation was observed between c-Kit mutations and tumour differentiation, mitotic activity or survival.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esplênicas
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Mastocitose
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Doenças do Gato
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Receptores Proteína Tirosina Quinases
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Proteínas Proto-Oncogênicas c-kit
Limite:
Animals
Idioma:
En
Revista:
Vet Comp Oncol
Assunto da revista:
MEDICINA VETERINARIA
/
NEOPLASIAS
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Itália