Nano-TiO2 penetration of oral mucosa: in vitro analysis using 3D organotypic human buccal mucosa models.
J Oral Pathol Med
; 46(3): 214-222, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-27387227
ABSTRACT
BACKGROUND:
Oral cavity is a doorway for a variety of products containing titanium dioxide (TiO2 ) nanoparticles (NPs) (nano-TiO2 ) such as food additives, oral healthcare products and dental materials. Their potential to penetrate and affect normal human oral mucosa is not yet determined.OBJECTIVES:
To evaluate the ability of nano-TiO2 to penetrate the in vitro reconstructed normal human buccal mucosa (RNHBM).METHODS:
RNHBM was generated from primary normal human oral keratinocytes and fibroblasts isolated from buccal oral mucosa of healthy patients (n = 6). The reconstructed tissues were exposed after 10 days to clinically relevant concentrations of spherical or spindle rutile nano-TiO2 in suspension for short (20 min) and longer time (24 h). Ultrahigh-resolution imaging (URI) microscopy (CytoViva™ , Auburn, AL, USA) was used to assess the depth of penetration into reconstructed tissues.RESULTS:
Ultrahigh-resolution imaging microscopy demonstrated the presence of nano-TiO2 mostly in the epithelium of RNHBM at both 20 min and 24-h exposure, and this was shape and doze dependent at 24 h of exposure. The depth of penetration diminished in time at higher concentrations. The exposed epithelium showed increased desquamation but preserved thickness.CONCLUSION:
Nano-TiO2 is able to penetrate RNHBM and to activate its barrier function in a doze- and time-dependent manner.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Titânio
/
Mucosa Bucal
Limite:
Humans
Idioma:
En
Revista:
J Oral Pathol Med
Assunto da revista:
ODONTOLOGIA
/
PATOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Noruega