HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme.
Biosci Biotechnol Biochem
; 62(8): 1643-6, 1998.
Article
em En
| MEDLINE
| ID: mdl-27388851
The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated α and ß anomeric forms of N-acetylchitooligosaccharides, and obtain the following advantages of this HPLC method. 1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing α anomer formation from the retaining mechanism showing ß anomer formation. 2. We can also estimate the cleavage patterns of N-acetylchitooligosaccharides by chitinolytic enzymes by using natural substrates.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
1998
Tipo de documento:
Article