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HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme.
Koga, D; Yoshioka, T; Arakane, Y.
Afiliação
  • Koga D; a Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University.
  • Yoshioka T; a Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University.
  • Arakane Y; b Research Laboratory.
Biosci Biotechnol Biochem ; 62(8): 1643-6, 1998.
Article em En | MEDLINE | ID: mdl-27388851
The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated α and ß anomeric forms of N-acetylchitooligosaccharides, and obtain the following advantages of this HPLC method. 1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing α anomer formation from the retaining mechanism showing ß anomer formation. 2. We can also estimate the cleavage patterns of N-acetylchitooligosaccharides by chitinolytic enzymes by using natural substrates.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 1998 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 1998 Tipo de documento: Article