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Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples.
Scoleri, Gianny P; Choo, Jocelyn M; Leong, Lex E X; Goddard, Thomas R; Shephard, Lisa; Burr, Lucy D; Bastian, Ivan; Thomson, Rachel M; Rogers, Geraint B.
Afiliação
  • Scoleri GP; SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
  • Choo JM; SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
  • Leong LE; SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
  • Goddard TR; Department of Respiratory and Medicine, WCH, and Paediatrics and Reproductive Health, School of Medicine, University of Adelaide, Australia.
  • Shephard L; Microbiology Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia.
  • Burr LD; Immunity, Infection and Inflammation Program, Mater Research Institute, University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia Department of Respiratory Medicine, Mater Health Services, South Brisbane, QLD, Australia.
  • Bastian I; Microbiology Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia.
  • Thomson RM; Gallipoli Medical Research Centre, Greenslopes Private Hospital and University of Queensland, Brisbane, Queensland, Australia.
  • Rogers GB; SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia geraint.rogers@sahmri.com.
J Clin Microbiol ; 54(9): 2395-8, 2016 09.
Article em En | MEDLINE | ID: mdl-27413194
ABSTRACT
Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Técnicas de Diagnóstico Molecular / Reação em Cadeia da Polimerase em Tempo Real / Micobactérias não Tuberculosas / Infecções por Mycobacterium não Tuberculosas Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções Respiratórias / Técnicas de Diagnóstico Molecular / Reação em Cadeia da Polimerase em Tempo Real / Micobactérias não Tuberculosas / Infecções por Mycobacterium não Tuberculosas Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália