Monitoring of the Binding Between EGFR Protein and EGFR Aptamer Using In-Situ Total Internal Reflection Ellipsometry.
J Nanosci Nanotechnol
; 16(6): 6445-9, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27427733
ABSTRACT
The epidermal growth factor receptor (EGFR) protein has received significant attention in medical biotechnology because it is an important component in cell growth and division. We report the results of a study on the binding between the EGFR protein and the associated aptamer, measured in real time. Aptamers can be used for clinical purposes including macromolecular medicine and basic research. In particular, EGFR aptamers are promising molecular agents for targeting cancer. The data were obtained in-situ with total internal reflection ellipsometry (TIRE), which combines the analytic capability of spectroscopic ellipsometry with the high surface sensitivity of surface plasmon resonance measurements. Our results show that TIRE can be used to determine adsorption of nanoscale biomolecules. Our results are supported by additional data obtained by liquid atomic-force-microscopy.
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Base de dados:
MEDLINE
Assunto principal:
Ressonância de Plasmônio de Superfície
/
Aptâmeros de Nucleotídeos
/
Fenômenos Ópticos
/
Receptores ErbB
Limite:
Humans
Idioma:
En
Revista:
J Nanosci Nanotechnol
Ano de publicação:
2016
Tipo de documento:
Article