Simple and efficient ultrafiltration method for purification of rotavirus VP6 oligomeric proteins.
Arch Virol
; 161(11): 3219-23, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-27518400
ABSTRACT
Bacterial endotoxins, DNA, live viruses, and viral proteins derived from bacterial and baculovirus (BV) expression vectors employed in recombinant protein production contaminate the final product. Density gradient centrifugation is commonly used to partially purify oligomeric proteins, but impurities from the expression system still remain. We describe a simple and rapid ultrafiltration method for final purification of rotavirus VP6 oligomeric nanotubes and nanospheres. Contamination originating from the BV vector used in VP6 production was undetectable. The method is highly efficient, fast and inexpensive and can be used for a small-scale laboratory purification of VP6 protein to replace technically demanding multi-step chromatographic procedures.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Virologia
/
Proteínas Recombinantes
/
Ultrafiltração
/
Proteínas do Capsídeo
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Antígenos Virais
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Finlândia