Assessment of direct versus indirect magnetic bead-based T-cell isolation procedures followed by magnetic bead-based DNA isolation.
Lupus Sci Med
; 3(1): e000167, 2016.
Article
em En
| MEDLINE
| ID: mdl-27547441
ABSTRACT
OBJECTIVE:
To compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation.METHODS:
T-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assessed.RESULTS:
Direct and indirect labelling resulted in comparable T-cell purity (93.11±1.47% vs. 94.99±1.54%, p= 0.125) and DNA concentration per cell (50.97±14.15 ng/(mlxcell) vs. 49.53±13.62 ng/(mlxcell), p=0.492), while DNA purity was significantly higher after direct labelling (1.82±0.05 vs. 1.78±0.03, p=0.0488).CONCLUSIONS:
Both direct and indirect magnetic bead-based T-cell selection may be used prior to magnetic bead-based DNA isolation procedures.
Texto completo:
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Lupus Sci Med
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha