Production and secretion of recombinant soluble CD3 polypeptides by myeloma-derived transfectant clones.
Immunol Lett
; 21(3): 243-8, 1989 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-2767725
ABSTRACT
Soluble forms of three human CD3 proteins have been produced by recombinant DNA techniques. The extracellular domain of CD3-gamma, -delta or -epsilon has been linked to the constant region of mouse immunoglobulin kappa light chain to form gamma-kappa, delta-kappa and epsilon-kappa chimaeric proteins. These are secreted by mouse myeloma-derived transfectant cell lines and are immunoprecipitable by CD3- or kappa-specific polyclonal antisera. Yields of 100-500 micrograms secreted recombinant proteins per litre of culture medium were obtained, which could be purified by anti-kappa affinity chromatography. The production of soluble CD3 illustrates the applicability of this technology to a loosely associated protein complex.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Transfecção
/
Antígenos de Diferenciação
Idioma:
En
Revista:
Immunol Lett
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Suíça