Distribution, position and genomic characteristics of crossovers in tomato recombinant inbred lines derived from an interspecific cross between Solanum lycopersicum and Solanum pimpinellifolium.
Plant J
; 89(3): 554-564, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-27797425
ABSTRACT
We determined the crossover (CO) distribution, frequency and genomic sequences involved in interspecies meiotic recombination by using parent-assigned variants of 52 F6 recombinant inbred lines obtained from a cross between tomato, Solanum lycopersicum, and its wild relative, Solanum pimpinellifolium. The interspecific CO frequency was 80% lower than reported for intraspecific tomato crosses. We detected regions showing a relatively high and low CO frequency, so-called hot and cold regions. Cold regions coincide to a large extent with the heterochromatin, although we found a limited number of smaller cold regions in the euchromatin. The CO frequency was higher at the distal ends of chromosomes than in pericentromeric regions and higher in short arm euchromatin. Hot regions of CO were detected in euchromatin, and COs were more often located in non-coding regions near the 5' untranslated region of genes than expected by chance. Besides overrepresented CCN repeats, we detected poly-A/T and AT-rich motifs enriched in 1-kb promoter regions flanking the CO sites. The most abundant sequence motifs at CO sites share weak similarity to transcription factor-binding sites, such as for the C2H2 zinc finger factors class and MADS box factors, while InterPro scans detected enrichment for genes possibly involved in the repair of DNA breaks.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Solanum lycopersicum
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Genoma de Planta
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Troca Genética
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Cromossomos de Plantas
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Solanum
Idioma:
En
Revista:
Plant J
Assunto da revista:
BIOLOGIA MOLECULAR
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BOTANICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Holanda