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Multidimensional chemical control of CRISPR-Cas9.
Maji, Basudeb; Moore, Christopher L; Zetsche, Bernd; Volz, Sara E; Zhang, Feng; Shoulders, Matthew D; Choudhary, Amit.
Afiliação
  • Maji B; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Moore CL; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
  • Zetsche B; Renal Division, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Volz SE; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Zhang F; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Shoulders MD; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
  • Choudhary A; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Nat Chem Biol ; 13(1): 9-11, 2017 Jan.
Article em En | MEDLINE | ID: mdl-27820801
ABSTRACT
Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos