NADPH oxidase-dependent degradation of single-walled carbon nanotubes in macrophages.
J Mater Sci Mater Med
; 28(1): 7, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-27885574
ABSTRACT
Previous studies have shown that carboxylated single-walled carbon nanotubes (SWCNTs) could be oxidatively biodegraded by neutrophil myeloperoxidase (MPO) and peroxynitrite (ONOO-). However, the biodegradation mechanism of nanotubes in macrophages has not been explored enough. Here, we showed that both MPO and ONOO- could effectively oxidize SWCNTs to generate shorter and oxidative nanotubes in vitro. SWCNTs were significantly degraded in zymosan-stimulated macrophages, and the degradation mechanism was dependent on MPO and ONOO--driven oxidative pathways of activated macrophages, where NADPH oxidase was found to be a major determinant of the biodegradation process. Moreover, the functionalization of IgG to SWCNTs could stimulate MPO release and ONOO- formation in macrophages, thereby creating the conditions favorable for degradation of nanotubes and subsequently contributing to the higher degradation degree of IgG-coated SWCNTs. Therefore, our discovery of NADPH oxidase-dependent SWCNTs degradation in activated macrophages will open new opportunities for the regulation of SWCNTs fate in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Peroxidase
/
NADPH Oxidases
/
Ácido Peroxinitroso
/
Nanotubos de Carbono
Limite:
Humans
Idioma:
En
Revista:
J Mater Sci Mater Med
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China