Evaluation of the in vivo mutagenicity of melamine by the RBC Pig-a assay and PIGRET assay.
Mutat Res Genet Toxicol Environ Mutagen
; 811: 43-48, 2016 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-27931813
ABSTRACT
The Pig-a assay is a new in vivo genotoxicity test for detecting mutagens in the bodies of animals, using the endogenous Pig-a gene as the target. There are two types of Pig-a assays the red blood cell (RBC) Pig-a assay, which uses RBCs, and the PIGRET assay, which uses reticulocytes. The Japanese Environmental Mutagen Society-Mammalian Mutagenicity Study Group collaborative study of the Pig-a assay was carried out to investigate the usefulness of the PIGRET assay. The mutagenicity of melamine was evaluated as part of this study. Eight-week-old male CrlCD (SD) rats were administered a single gavage dose of melamine as a non-genotoxic bladder carcinogen. Blood samples were collected at the first, second and fourth weeks after administration, and the RBC Pig-a assay and PIGRET assays were conducted using these samples. Three dose levels were used in the study the highest dose was 2000mg/kg, which is generally used as the maximum dose in in vivo genotoxicity testing, and 1000 and 500mg/kg were also used. As a positive control, a group of rats was administered a single dose of N-nitroso-N-ethylurea (ENU) by gavage at 40mg/kg. The Pig-a mutant frequencies (Pig-a MFs) did not increase in any of the melamine groups throughout the experimental period in either the RBC Pig-a assay or the PIGRET assay. Both the RBC Pig-a and PIGRET assays revealed significant increases in the Pig-a MFs in the ENU group, starting at day 7 after a single administration. Therefore, these two assays, when evaluated after a single administration, can be used to determine that melamine is non-mutagenic.
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MEDLINE
Assunto principal:
Reticulócitos
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Triazinas
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Eritrócitos
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Proteínas de Membrana
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Testes de Mutagenicidade
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Mutat Res Genet Toxicol Environ Mutagen
Ano de publicação:
2016
Tipo de documento:
Article