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Structural enzymology using X-ray free electron lasers.
Kupitz, Christopher; Olmos, Jose L; Holl, Mark; Tremblay, Lee; Pande, Kanupriya; Pandey, Suraj; Oberthür, Dominik; Hunter, Mark; Liang, Mengning; Aquila, Andrew; Tenboer, Jason; Calvey, George; Katz, Andrea; Chen, Yujie; Wiedorn, Max O; Knoska, Juraj; Meents, Alke; Majriani, Valerio; Norwood, Tyler; Poudyal, Ishwor; Grant, Thomas; Miller, Mitchell D; Xu, Weijun; Tolstikova, Aleksandra; Morgan, Andrew; Metz, Markus; Martin-Garcia, Jose M; Zook, James D; Roy-Chowdhury, Shatabdi; Coe, Jesse; Nagaratnam, Nirupa; Meza, Domingo; Fromme, Raimund; Basu, Shibom; Frank, Matthias; White, Thomas; Barty, Anton; Bajt, Sasa; Yefanov, Oleksandr; Chapman, Henry N; Zatsepin, Nadia; Nelson, Garrett; Weierstall, Uwe; Spence, John; Schwander, Peter; Pollack, Lois; Fromme, Petra; Ourmazd, Abbas; Phillips, George N; Schmidt, Marius.
Afiliação
  • Kupitz C; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Olmos JL; Department of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
  • Holl M; Department of Physics, Arizona State University , Tempe, Arizona 85287, USA.
  • Tremblay L; Marbles Inc. , 1900 Belvedere Pl, Westfield, Indiana 46074, USA.
  • Pandey S; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Hunter M; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Liang M; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Aquila A; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory , 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Tenboer J; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Calvey G; Department of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
  • Katz A; Department of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
  • Chen Y; Department of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
  • Wiedorn MO; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Knoska J; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Meents A; University of Hamburg , Luruper Chaussee 149, 22761 Hamburg, Germany.
  • Norwood T; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Poudyal I; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Grant T; Hauptman-Woodward Institute, State University of New York at Buffalo , 700 Ellicott Street, Buffalo, New York 14203, USA.
  • Miller MD; Department of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
  • Xu W; Department of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
  • Tolstikova A; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Morgan A; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Metz M; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Martin-Garcia JM; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Zook JD; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Roy-Chowdhury S; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Coe J; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Nagaratnam N; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Meza D; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Fromme R; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Basu S; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Frank M; Lawrence Livermore National Laboratory , Livermore, California 94550, USA.
  • White T; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Barty A; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Bajt S; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Yefanov O; Center for Free-Electron Laser Science, DESY , Notkestrasse 85, 22607 Hamburg, Germany.
  • Zatsepin N; Department of Physics, Arizona State University , Tempe, Arizona 85287, USA.
  • Nelson G; Department of Physics, Arizona State University , Tempe, Arizona 85287, USA.
  • Weierstall U; Department of Physics, Arizona State University , Tempe, Arizona 85287, USA.
  • Spence J; Department of Physics, Arizona State University , Tempe, Arizona 85287, USA.
  • Schwander P; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Pollack L; Department of Applied and Engineering Physics, Cornell University , 254 Clark Hall, Ithaca, New York 14853, USA.
  • Fromme P; School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University , Tempe, Arizona 85287-1604, USA.
  • Ourmazd A; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
  • Phillips GN; Department of BioSciences, Rice University , 6100 Main Street, Houston, Texas 77005, USA.
  • Schmidt M; Physics Department, University of Wisconsin-Milwaukee , 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Struct Dyn ; 4(4): 044003, 2017 Jul.
Article em En | MEDLINE | ID: mdl-28083542
ABSTRACT
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated. Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction of M. tuberculosis ß-lactamase microcrystals with ceftriaxone antibiotic solution. Electron density maps of the apo-ß-lactamase and of the ceftriaxone bound form were obtained at 2.8 Å and 2.4 Å resolution, respectively. These results pave the way to study cyclic and non-cyclic reactions and represent a new field of time-resolved structural dynamics for numerous substrate-triggered biological reactions.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Struct Dyn Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Struct Dyn Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos