Characterization of mitochondrial DNA polymorphisms in the Han population in Liaoning Province, Northeast China.
Mitochondrial DNA A DNA Mapp Seq Anal
; 29(2): 250-255, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-28093929
ABSTRACT
This study characterized the genetic variations of mitochondrial DNA (mtDNA) to elucidate the maternal genetic structure of Liaoning Han Chinese. A total of 317 blood samples of unrelated individuals were collected for analysis in Liaoning Province. The mtDNA samples were analyzed using two distinct methods:
sequencing of the hypervariable sequences I and II (HVSI and HVSII), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the coding region. The results indicated a high gene diversity value (0.9997 ± 0.0003), a high polymorphism information content (0.99668) and a random match probability (0.00332). These samples were classified into 305 haplotypes, with 9 shared haplotypes. The most common haplogroup was D4 (12.93%). The principal component analysis map, the phylogenetic tree map, and the genetic distance matrix all indicated that the genetic distance of the Liaoning Han population from the Tibetan group was distant, whereas that from the Miao group was relatively close.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
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Polimorfismo de Nucleotídeo Único
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Povo Asiático
Limite:
Female
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Humans
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Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Mitochondrial DNA A DNA Mapp Seq Anal
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China