Molecular Identification of Malassezia Species in Patients with Malassezia folliculitis in Sfax, Tunisia.
Mycopathologia
; 182(5-6): 583-589, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28108869
ABSTRACT
AIM:
Malassezia folliculitis is caused by the invasion of hair follicles by large numbers of Malassezia cells. Several Malassezia researches still use cultures, morphology and biochemical techniques. The aim of this study was to identify Malassezia species isolated from patients diagnosed with folliculitis, at the Parasitology and Mycology Laboratory of Sfax University Hospital, and to explore the genetic diversity of Malassezia by using PCR-RFLP and PCR-sequencing targeting the rDNA region of the Malassezia genome. PATIENTS ANDMETHODS:
Specimens were taken from 27 patients with Malassezia folliculitis. For the molecular identification, PCR amplification of the 26S rDNAD1/D2 region was carried out using the Malup and Maldown primers and three restriction enzymes (BanI, MspI and HeaII) for RFLP analysis. The nucleotide sequences of each isolate were compared to those in the NCBI GenBank by using BLASTIN algorithm.RESULTS:
Three species of Malassezia yeasts were identified among the 31 Malassezia strains isolated M. globosa (83.9%), M. sympodialis (12. 9%) and M. furfur (3.2%). The sequence analysis of M. globosa showed six genotypes.CONCLUSION:
There is a high genotypic variability of M. globosa colonizing patients with folliculitis.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Dermatomicoses
/
Foliculite
/
Malassezia
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
Africa
Idioma:
En
Revista:
Mycopathologia
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Tunísia