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Colorimetric detection of Cucumber green mottle mosaic virus using unmodified gold nanoparticles as colorimetric probes.
Wang, Lin; Liu, Zhanmin; Xia, Xueying; Yang, Cuiyun; Huang, Junyi; Wan, Sibao.
Afiliação
  • Wang L; School of Life Sciences, Shanghai University, Shanghai, 200444, China.
  • Liu Z; School of Life Sciences, Shanghai University, Shanghai, 200444, China. Electronic address: zhmliu@shu.edu.cn.
  • Xia X; School of Life Sciences, Shanghai University, Shanghai, 200444, China.
  • Yang C; Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China.
  • Huang J; School of Life Sciences, Shanghai University, Shanghai, 200444, China.
  • Wan S; School of Life Sciences, Shanghai University, Shanghai, 200444, China.
J Virol Methods ; 243: 113-119, 2017 05.
Article em En | MEDLINE | ID: mdl-28109844
Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction. Normally, species-specific probes attach to the surface of AuNPs and thereby increasing their resistance to NaCl-induced aggregation. The concentration of sodium, probes in the reaction system and evaluation of specificity and sensitivity of a novel assay, visual detection of Cucumber green mottle mosaic virus using unmodified AuNPs has been carried out with simple preparation of samples in our study. Through this assay, as low as 30pg/µL of CGMMV RNA was thus detected visually, by the naked eye, without the need for any sophisticated, expensive instrumentation and biochemical reagents. The specificity was 100% and exhibited good reproducibility in our assays. The results note that this assay is highly species-specific, simple, low-cost, and visual for easy detection of CGMMV in plant tissues. Therefore, visual assay is a potentially useful tool for middle or small-scales corporations and entry-exit inspection and quarantine bureau to detect CGMMV in cucumber seeds or plant tissues.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / RNA Viral / Tobamovirus / Colorimetria / Nanopartículas / Ouro Tipo de estudo: Diagnostic_studies / Evaluation_studies Idioma: En Revista: J Virol Methods Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doenças das Plantas / RNA Viral / Tobamovirus / Colorimetria / Nanopartículas / Ouro Tipo de estudo: Diagnostic_studies / Evaluation_studies Idioma: En Revista: J Virol Methods Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China