From Optimization to Mapping: An Evolutionary Algorithm for Protein Energy Landscapes.
IEEE/ACM Trans Comput Biol Bioinform
; 15(3): 719-731, 2018.
Article
em En
| MEDLINE
| ID: mdl-28113951
ABSTRACT
Stochastic search is often the only viable option to address complex optimization problems. Recently, evolutionary algorithms have been shown to handle challenging continuous optimization problems related to protein structure modeling. Building on recent work in our laboratories, we propose an evolutionary algorithm for efficiently mapping the multi-basin energy landscapes of dynamic proteins that switch between thermodynamically stable or semi-stable structural states to regulate their biological activity in the cell. The proposed algorithm balances computational resources between exploration and exploitation of the nonlinear, multimodal landscapes that characterize multi-state proteins via a novel combination of global and local search to generate a dynamically-updated, information-rich map of a protein's energy landscape. This new mapping-oriented EA is applied to several dynamic proteins and their disease-implicated variants to illustrate its ability to map complex energy landscapes in a computationally feasible manner. We further show that, given the availability of such maps, comparison between the maps of wildtype and variants of a protein allows for the formulation of a structural and thermodynamic basis for the impact of sequence mutations on dysfunction that may prove useful in guiding further wet-laboratory investigations of dysfunction and molecular interventions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Algoritmos
/
Proteínas
/
Biologia Computacional
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
ACM Trans Comput Biol Bioinform
Assunto da revista:
BIOLOGIA
/
INFORMATICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article