Chondrotoxicity of Liposomal Bupivacaine in Articular Chondrocytes: Preliminary Findings.
Mil Med
; 182(S1): 185-188, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28291471
ABSTRACT
OBJECTIVE:
The chondrotoxicity of local anesthetics has been previously recognized. Recent introduction of a liposomal formulation of bupivacaine has been found to significantly improve postoperative pain control but its effect on chondrocyte viability has yet to be investigated with this new formulation. We sought to assess the in vitro chondrotoxicity of liposomal bupivacaine.METHODS:
Chondrocytes were isolated from articular cartilage from fresh stifle joints and grown in culture medium. Cultured chondrocyte-derived cells (CDCs) were treated with 0.9% normal saline solution, 0.5%, 0.25%, and 0.13% bupivacaine and ropivacaine, 1.3% liposomal bupivacaine for 1 hour. Following treatment, cells were washed and incubated in media for 23 hours. The CDCs were then harvested and viability was assessed by flow cytometry using SYTOX green dead cell stain.RESULTS:
Treated CDCs demonstrated a dose-response effect for chondrocyte viability when treated with bupivacaine, ropivacaine, and liposomal bupivacaine. Liposomal bupivacaine demonstrated the highest chondrocyte viability following treatment. Ropivacaine demonstrated higher chondrocyte viability than bupivacaine.CONCLUSION:
Following 1 hour of treatment, liposomal bupivacaine demonstrated the highest chondrocyte viability. Chondrocyte viability was inversely proportional to anesthetic concentration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bupivacaína
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Resultado do Tratamento
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Morte Celular
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Condrócitos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mil Med
Ano de publicação:
2017
Tipo de documento:
Article