Characterization of the transformation properties of Shope fibroma virus.
Virus Res
; 9(1): 33-48, 1988 Jan.
Article
em En
| MEDLINE
| ID: mdl-2829464
ABSTRACT
To investigate if Shope fibroma virus (SFV), a leporipoxvirus that induces benign tumors in adult rabbits, can trigger the second step of carcinogenesis in vitro or malignant transformation, an already immortalized rabbit cell line (SIRC) was inoculated with ultraviolet-irradiated virus. The resulting cell transformants displayed the characteristic properties of the malignant phenotype lack of infectious particles, low serum requirement, high efficiency of cloning, resistance to superinfection, presence of viral DNA sequences in the nucleus, expression of viral proteins and induction of tumors in rabbits. However, this transformation was not stable since in all cell lines studied, a loss of the malignant phenotype was recorded close to the 50th passage. To assess the oncogenic potential of SFV, NIH 3T3 cells were transfected with SFV DNA. The results of these experiments indicate that SFV DNA can induce the formation of foci in certain NIH 3T3 cell lines. Taken together these results support the notion that SFV can elicit the transformation of cells in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Poxviridae
/
Transformação Celular Neoplásica
/
Transformação Celular Viral
/
Vírus do Fibroma dos Coelhos
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Virus Res
Assunto da revista:
VIROLOGIA
Ano de publicação:
1988
Tipo de documento:
Article