Widespread promoter methylation of synaptic plasticity genes in long-term potentiation in the adult brain in vivo.
BMC Genomics
; 18(1): 250, 2017 03 23.
Article
em En
| MEDLINE
| ID: mdl-28335720
ABSTRACT
BACKGROUND:
DNA methylation is a key modulator of gene expression in mammalian development and cellular differentiation, including neurons. To date, the role of DNA modifications in long-term potentiation (LTP) has not been explored.RESULTS:
To investigate the occurrence of DNA methylation changes in LTP, we undertook the first detailed study to describe the methylation status of all known LTP-associated genes during LTP induction in the dentate gyrus of live rats. Using a methylated DNA immunoprecipitation (MeDIP)-array, together with previously published matched RNA-seq and public histone modification data, we discover widespread changes in methylation status of LTP-genes. We further show that the expression of many LTP-genes is correlated with their methylation status. We show that these correlated genes are enriched for RNA-processing, active histone marks, and specific transcription factors. These data reveal that the synaptic activity-evoked methylation changes correlates with pre-existing activation of the chromatin landscape. Finally, we show that methylation of Brain-derived neurotrophic factor (Bdnf) CpG-islands correlates with isoform switching from transcripts containing exon IV to exon I.CONCLUSIONS:
Together, these data provide the first evidence of widespread regulation of methylation status in LTP-associated genes.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Regiões Promotoras Genéticas
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Potenciação de Longa Duração
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Metilação de DNA
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Plasticidade Neuronal
Limite:
Adult
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Humans
Idioma:
En
Revista:
BMC Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Austrália