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Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution.
Kuevda, E V; Gubareva, E A; Gumenyuk, I S; Sotnichenko, A S; Gilevich, I V; Nakokhov, R Z; Rusinova, T V; Yudina, T G; Red'ko, A N; Alekseenko, S N.
Afiliação
  • Kuevda EV; Kuban State Medical University, Krasnodar, Russia. elenakuevda@yandex.ru.
  • Gubareva EA; Kuban State Medical University, Krasnodar, Russia.
  • Gumenyuk IS; Kuban State Medical University, Krasnodar, Russia.
  • Sotnichenko AS; Kuban State Medical University, Krasnodar, Russia.
  • Gilevich IV; Kuban State Medical University, Krasnodar, Russia.
  • Nakokhov RZ; Kuban State Medical University, Krasnodar, Russia.
  • Rusinova TV; Kuban State Medical University, Krasnodar, Russia.
  • Yudina TG; Kuban State Medical University, Krasnodar, Russia.
  • Red'ko AN; Kuban State Medical University, Krasnodar, Russia.
  • Alekseenko SN; Kuban State Medical University, Krasnodar, Russia.
Bull Exp Biol Med ; 162(5): 703-706, 2017 Mar.
Article em En | MEDLINE | ID: mdl-28361431
We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alicerces Teciduais / Pulmão Limite: Animals Idioma: En Revista: Bull Exp Biol Med Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Federação Russa
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Alicerces Teciduais / Pulmão Limite: Animals Idioma: En Revista: Bull Exp Biol Med Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Federação Russa