Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury.
Korean Circ J
; 47(2): 193-200, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-28382074
ABSTRACT
BACKGROUND AND OBJECTIVES:
The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. MATERIALS ANDMETHODS:
We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial H2O2 and mitochondrial complex activity.RESULTS:
KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress.CONCLUSION:
KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Korean Circ J
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China