Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence.
Anal Chem
; 89(8): 4363-4366, 2017 04 18.
Article
em En
| MEDLINE
| ID: mdl-28382823
ABSTRACT
New approaches for genomic DNA/RNA detection are in high demand in order to provide controls for existing enzymatic technologies and to create alternatives for emerging applications. In particular, there is an unmet need in rapid, reliable detection of short RNA regions which could open up new opportunities in transcriptome analysis, virology, and other fields. Herein, we report for the first time a "click" chemistry approach to oligonucleotide probe elongation as a novel approach to specifically detect a viral sequence. We hybridized a library of short, terminally labeled probes to Ebola virus RNA followed by click assembly and analysis of the read sequence by various techniques. As we demonstrate in this paper, using our new approach, a viral RNA sequence can be detected in less than 2 h without the need for cDNA synthesis or any other enzymatic reactions and with a sensitivity of <10 pM target RNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Sondas de Oligonucleotídeos
/
Ebolavirus
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Dinamarca