Eomes expression reports the progressive differentiation of IFN-γ-producing Th1-like γδ T cells.
Eur J Immunol
; 47(6): 970-981, 2017 06.
Article
em En
| MEDLINE
| ID: mdl-28386934
ABSTRACT
The transcription factor Eomesodermin (Eomes) plays a crucial role in regulating cytotoxic function, development, and survival of immune cells. γδ T cells can express Eomes, but its contribution to their differentiation is unknown. Using Eomes-IRES-GFP mice, we show that Eomes+ γδ T cells are unequally distributed among organs, with the highest proportion in spleen. While the majority of Eomes+ γδ T cells expressed Vγ1+ and Vγ4+ TCRs, Eomes was absent in Vγ5+ , Vγ6+ , and Vγ7+ subsets. Moreover, Eomes was co-expressed in γδ T cells with Th1 lineage-related factors such as CD27, T-bet, and Ly6C, but not with Th17 lineage-related genes. Eomes+ and Eomes- γδ T-cell populations showed distinct gene expression profiles, with an increase of cytotoxic-related genes in Eomes+ γδ T cells. Furthermore, Eomes could be induced in peripheral γδ T cells by IL-12 and IL-4, and Eomes+ γδ T cells presented a higher proliferation rate and IFN-γ production when stimulated in vitro with IL-12 and IL-18. However, γδ T cells with very high Eomes levels displayed an exhausted phenotype with high levels of PD-1, and were less capable of IFN-γ production. Together, this study highlights Eomes as a marker for the differentiation of Th1-like effector γδ T cells.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Interferon gama
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Receptores de Antígenos de Linfócitos T gama-delta
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Células Th1
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Proteínas com Domínio T
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha