Identification and characterization of the CRPV E7 protein expressed in COS-7 cells.
Virology
; 165(1): 134-40, 1988 Jul.
Article
em En
| MEDLINE
| ID: mdl-2838953
ABSTRACT
The papillomavirus E7 protein may play an important role in oncogenesis as it is the major viral protein present in human cervical cancer-derived cell lines. Because of the relevance of the cottontail rabbit papillomavirus (CRPV) system for the study of viral-induced malignancies, we characterized its E7 protein expressed by a heterologous strong promoter. An E7-specific antiserum was obtained by immunizing rabbits with a Trp E-E7 fusion protein containing the 88 carboxyl-terminal amino acids of E7. This antiserum specifically precipitated from [35S]cysteine but not from 32PO4-labeled transiently transfected COS-7 cells a 14-kDa protein. The protein was present only in the soluble cytoplasmic fraction and exhibited a heterogeneous sedimentation rate in nondenaturing glycerol gradients.
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Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Proteínas Virais
/
Papillomavirus de Coelho Cottontail
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
1988
Tipo de documento:
Article