FunChIP: an R/Bioconductor package for functional classification of ChIP-seq shapes.
Bioinformatics
; 33(16): 2570-2572, 2017 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-28398543
ABSTRACT
SUMMARY:
Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) generates local accumulations of sequencing reads on the genome ("peaks"), which correspond to specific protein-DNA interactions or chromatin modifications. Peaks are detected by considering their total area above a background signal, usually neglecting their shapes, which instead may convey additional biological information. We present FunChIP, an R/Bioconductor package for clustering peaks according to a functional representation of their shapes after approximating their profiles with cubic B-splines, FunChIP minimizes their functional distance and classifies the peaks applying a k-mean alignment and clustering algorithm. The whole pipeline is user-friendly and provides visualization functions for a quick inspection of the results. An application to the transcription factor Myc in 3T9 murine fibroblasts shows that clusters of peaks with different shapes are associated with different genomic locations and different transcriptional regulatory activity. AVAILABILITY AND IMPLEMENTATION The package is implemented in R and is available under Artistic Licence 2.0 from the Bioconductor website (http//bioconductor.org/packages/FunChIP). CONTACT marco.morelli@iit.it. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Software
/
Genômica
/
Imunoprecipitação da Cromatina
Limite:
Animals
Idioma:
En
Revista:
Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Itália