Purification and crystallization of thymidine kinase from herpes simplex virus type 1.

Sanderson, M R; Freemont, P S; Murthy, H M; Krane, J F; Summers, W C; Steitz, T A

Sanderson, M R; Freemont, P S; Murthy, H M; Krane, J F; Summers, W C; Steitz, T A.

Afiliação

Sanderson MR; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CN 06511.

J Mol Biol ; 202(4): 917-9, 1988 Aug 20.

Article em En | MEDLINE | ID: mdl-2845104

ABSTRACT

ABSTRACT

Thymidine kinase from herpes simplex virus type 1 (ATP thymidine 5'-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4122 or P4322, and have unit cell dimensions a = b = 84 A, c = 180 A.

Assuntos

Simplexvirus/enzimologia; Timidina Quinase; Cristalização; Timidina Quinase/isolamento & purificação; Difração de Raios X

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Base de dados: MEDLINE Assunto principal: Timidina Quinase / Simplexvirus Idioma: En Revista: J Mol Biol Ano de publicação: 1988 Tipo de documento: Article

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Imprimir

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PubMed Links

Base de dados: MEDLINE Assunto principal: Timidina Quinase / Simplexvirus Idioma: En Revista: J Mol Biol Ano de publicação: 1988 Tipo de documento: Article