Frequency of Toxoplasma gondii in the retina in eye banks in Brazil.
Parasitol Res
; 116(7): 2031-2033, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28508165
ABSTRACT
Ocular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Toxoplasma
/
Bancos de Olhos
Limite:
Adult
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Animals
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Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Parasitol Res
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil