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Fungal Diversity in Field Mold-Damaged Soybean Fruits and Pathogenicity Identification Based on High-Throughput rDNA Sequencing.
Liu, Jiang; Deng, Jun-Cai; Yang, Cai-Qiong; Huang, Ni; Chang, Xiao-Li; Zhang, Jing; Yang, Feng; Liu, Wei-Guo; Wang, Xiao-Chun; Yong, Tai-Wen; Du, Jun-Bo; Shu, Kai; Yang, Wen-Yu.
Afiliação
  • Liu J; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Deng JC; Institute of Ecological Agriculture, Sichuan Agricultural UniversityChengdu, China.
  • Yang CQ; Sichuan Engineering Research Center for Crop Strip Intercropping SystemChengdu, China.
  • Huang N; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Chang XL; Sichuan Engineering Research Center for Crop Strip Intercropping SystemChengdu, China.
  • Zhang J; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Yang F; Institute of Ecological Agriculture, Sichuan Agricultural UniversityChengdu, China.
  • Liu WG; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Wang XC; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Yong TW; Institute of Ecological Agriculture, Sichuan Agricultural UniversityChengdu, China.
  • Du JB; Sichuan Engineering Research Center for Crop Strip Intercropping SystemChengdu, China.
  • Shu K; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
  • Yang WY; Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of AgricultureChengdu, China.
Front Microbiol ; 8: 779, 2017.
Article em En | MEDLINE | ID: mdl-28515718
Continuous rain and an abnormally wet climate during harvest can easily lead to soybean plants being damaged by field mold (FM), which can reduce seed yield and quality. However, to date, the underlying pathogen and its resistance mechanism have remained unclear. The objective of the present study was to investigate the fungal diversity of various soybean varieties and to identify and confirm the FM pathogenic fungi. A total of 62,382 fungal ITS1 sequences clustered into 164 operational taxonomic units (OTUs) with 97% sequence similarity; 69 taxa were recovered from the samples by internal transcribed spacer (ITS) region sequencing. The fungal community compositions differed among the tested soybeans, with 42 OTUs being amplified from all varieties. The quadratic relationships between fungal diversity and organ-specific mildew indexes were analyzed, confirming that mildew on soybean pods can mitigate FM damage to the seeds. In addition, four potentially pathogenic fungi were isolated from FM-damaged soybean fruits; morphological and molecular identification confirmed these fungi as Aspergillus flavus, A. niger, Fusarium moniliforme, and Penicillium chrysogenum. Further re-inoculation experiments demonstrated that F. moniliforme is dominant among these FM pathogenic fungi. These results lay the foundation for future studies on mitigating or preventing FM damage to soybean.
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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Front Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China