Peroxiredoxins are required for spindle assembly, chromosome organization, and polarization in mouse oocytes.
Biochem Biophys Res Commun
; 489(2): 193-199, 2017 07 22.
Article
em En
| MEDLINE
| ID: mdl-28552528
ABSTRACT
Peroxiredoxins (Prxs) are highly conserved antioxidant enzymes and are implicated in multiple biological processes; however, their function in oocyte meiosis has not been studied. Here we show that inhibition of Prx I and II results in spindle defects, chromosome disorganization, and impaired polarization in mouse oocytes. Prx I was specifically localized at the spindle, whereas Prx II was enriched at the oocyte cortex and chromosomes. Inhibition of Prx activity with conoidin A disturbed assembly of the microtubule organizing center (MTOC) through Aurora A regulation, leading to defects in spindle formation. Moreover, conoidin A impaired actin filament and cortical granule (CG) distribution, disrupting actin cap and CG formation, respectively. Conoidin A also increased DNA damage without significantly increasing reactive oxygen species (ROS) levels, suggesting that the effects of conoidin A on meiotic maturation are not likely associated with ROS scavenging pathways. Therefore, our data suggest that Prxs are required for spindle assembly, chromosome organization, and polarization during meiotic maturation.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Polaridade Celular
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Cromossomos de Mamíferos
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Peroxirredoxinas
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Fuso Acromático
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Coréia do Sul