Non-Linear Formation of EtG and FAEEs after Controlled Administration of Low to Moderate Doses of Ethanol.
Alcohol Alcohol
; 52(5): 587-594, 2017 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-28591763
ABSTRACT
AIMS:
Ethyl glucuronide (EtG) and fatty acid ethyl esters (FAEEs) are non-oxidative metabolites of alcohol that can be detected in conventional and non-conventional biological matrices for longer periods than alcohol. The aim was to describe the time courses of both biomarkers after ingestion of acute low-moderate doses of ethanol.METHODS:
The study design was double-blind, randomized, crossover and controlled with placebo. Participants were distributed in three different cohorts (a) Cohort-1 two doses of 18 and 30 g of ethanol and placebo were administered to 12 subjects; (b) Cohort-2 two doses of 6 and 12 g of ethanol and placebo were administered to six subjects and (c) Cohort-3 two doses of 24 and 42 g of ethanol and placebo were administered to six subjects. Each participant received two doses of ethanol and placebo. Plasma concentrations (0-6 h) of ethanol and specific FAEEs (palmitic, stearic, linoleic and oleic acid ethyl esters) and urinary concentrations of EtG (0-24 h) were measured.RESULTS:
A dose-dependent increase in blood ethanol concentrations was observed. EtG excretion and FAEEs plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. Area under the curve (AUC0-6h) of ethanol concentrations showed a linear trend with non-oxidative metabolites' concentrations.CONCLUSION:
The formation rate of ethanol non-oxidative biomarkers does not follow a linear trend, explained mainly by a disproportionate increase in AUC0-6h of ethanol concentrations in relation to dose. This observation should be taken into account when interpreting results in biological matrices in clinical and forensic settings. SHORTSUMMARY:
A double-blind, randomized, crossover and controlled study was conducted administering ethanol (6-42 g). Ethyl glucuronide (EtG) excretion and fatty acid ethyl esters (FAEEs) plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. This observation should be taken into account when interpreting biomarker concentrations in clinical settings.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Etanol
/
Ácidos Graxos
/
Glucuronatos
Tipo de estudo:
Clinical_trials
/
Prognostic_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Alcohol Alcohol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Espanha