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Investigation of rabies virus glycoprotein carboxyl terminus as an in vitro predictive tool of neurovirulence. A 3R approach.
Seo, Wonhyo; Prehaud, Christophe; Khan, Zakir; Sabeta, Claude; Lafon, Monique.
Afiliação
  • Seo W; OIE Rabies Reference Laboratory, ARC-Onderstepoort Veterinary Institute (ARC-OVI), Onderstepoort, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa; Institut Pasteur, CNRS, Unité de Neuroimmunologie Virale, Département de Vi
  • Prehaud C; Institut Pasteur, CNRS, Unité de Neuroimmunologie Virale, Département de Virologie, Paris, France.
  • Khan Z; Institut Pasteur, CNRS, Unité de Neuroimmunologie Virale, Département de Virologie, Paris, France.
  • Sabeta C; OIE Rabies Reference Laboratory, ARC-Onderstepoort Veterinary Institute (ARC-OVI), Onderstepoort, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa.
  • Lafon M; Institut Pasteur, CNRS, Unité de Neuroimmunologie Virale, Département de Virologie, Paris, France. Electronic address: monique.lafon@pasteur.fr.
Microbes Infect ; 19(9-10): 476-484, 2017.
Article em En | MEDLINE | ID: mdl-28602914
ABSTRACT
In the field of live viral vaccines production, there is an unmet need for in vitro tests complying a 3R approach (Refine, Replace and Reduce the use of animal experimentation) to replace the post-licensing safety tests currently assayed in animals. Here, we performed a pilot study evaluating whether virulence of rabies virus, RABV, can be forecast by an in vitro test of neurite outgrowth. The rationale to use neurite outgrowth as a read-out for this test is based on the salient property of the cytoplasmic domain of the G-protein (Cyto-G) of virulent RABV strains - not of attenuated RABV strains - to stimulate neurite outgrowth in vitro. We observed that neurite elongation triggered by the Cyto-Gs encoded by different RABV field isolates correlate with the distinct virulence scores obtained in a mouse model of experimental rabies. Our results cast the idea that it could be feasible to predict RABV virulence by testing the in vitro property of a RABV strain to promote neurite outgrowth without the use of animal experimentation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Vírus da Raiva / Proteínas Virais / Glicoproteínas / Neuritos / Alternativas aos Testes com Animais Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Microbes Infect Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fragmentos de Peptídeos / Vírus da Raiva / Proteínas Virais / Glicoproteínas / Neuritos / Alternativas aos Testes com Animais Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Microbes Infect Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article