Polydiacetylene/Anti-HBs Complexes for Visible and Fluorescent Detection of Hepatitisâ
B Surface Antigen on a Nitrocellulose Membrane.
Chem Asian J
; 12(16): 2033-2037, 2017 Aug 17.
Article
em En
| MEDLINE
| ID: mdl-28603850
ABSTRACT
The immunochromatographic assay (ICA) using a nitrocellulose (NC) membrane offers several advantages. This technique is a rapid and straightforward method in contrast to other immunoassays. Polydiacetylene (PDA) vesicles have unique optical properties, displaying red color and red fluorescence at the same time. In this system, red-phase PDA vesicles are used as a fluorescent dye as well as a surface for immobilized hepatitisâ
B surface antibody (HBsAb). PDA has a remarkable stability compared with other fluorescent dyes. In this study, the most suitable PDA/HBsAb complexes are introduced for detecting hepatitisâ
B surface antigen (HBsAg). Then, the PDA/HBsAb complexes affixed antibody is attached to NC membrane, which has two lines to confirm detection of HBsAg. The main advantage of this system is that the detection of HBsAg can be observed in both visible and fluorescent images due to the optical properties of polydiacetylene. Detection of HBsAg is observed up to 0.1â
ng mL-1 by fluorescent analysis and confirmed by red line on the NC membrane up to 1â
ng mL-1 (HBsAg) using the naked eye. Consequently, these results show that PDA/HBsAb complexes were successfully applied to ICA for the diagnosis of hepatitisâ
B.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetileno
/
Imunoensaio
/
Técnicas Biossensoriais
/
Colódio
/
Antígenos de Superfície da Hepatite B
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Chem Asian J
Ano de publicação:
2017
Tipo de documento:
Article