Mangiferin protects osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling.
Biochem Biophys Res Commun
; 491(3): 807-813, 2017 09 23.
Article
em En
| MEDLINE
| ID: mdl-28669729
ABSTRACT
Oxidative stress has currently been proposed as a risk factor associated with the development and proression of osteoporosis. In this study, we identify the effect of mangiferin (MAN) on apoptosis and differentiation of osteoblast-like MC3T3-E1 cells insulted by H2O2. We firstly found that MAN can promote cell proliferation of MC3T3-E1 cells in a time- and dose-dependent manner and stimulate the phosphorylation of ERK5. Cells were divided as five groups control, H2O2 (100 µM, control), H2O2 + MAN (5 µM), H2O2 + MAN (10 µM), and H2O2 + MAN (20 µM). MAN can significantly decrease H2O2-induced apoptosis and elevated ROS level of MC3T3-E1 cells. The expressions of caspase-3, caspase-9 and Bax/Bcl-2 were increased with H2O2 treatment, and MAN can reverse these changes. In addition, Nrf2 and its downstream target effectors (HO1, NQO1) were dramatically attenuated in MC3T3-E cells treatment with H2O2, while MAN can significantly increase the expression of Nrf2, HO1 and NQO1. The expression of ERK5 was down regulated by RNA interference in MC3T3-E1 cells, and we found that MAN (20 µM) pretreatment didn't make remarkable decrease in cell apoptosis or expressions of apoptosis-related proteins in H2O2-insulted siRNA-ERK5 cells. This study indicated that MAN can protect osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling, which can be new agent for osteoporosis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Apoptose
/
Estresse Oxidativo
/
Xantonas
/
Peróxido de Hidrogênio
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China