Rapid identification of genes controlling virulence and immunity in malaria parasites.
PLoS Pathog
; 13(7): e1006447, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28704525
ABSTRACT
Identifying the genetic determinants of phenotypes that impact disease severity is of fundamental importance for the design of new interventions against malaria. Here we present a rapid genome-wide approach capable of identifying multiple genetic drivers of medically relevant phenotypes within malaria parasites via a single experiment at single gene or allele resolution. In a proof of principle study, we found that a previously undescribed single nucleotide polymorphism in the binding domain of the erythrocyte binding like protein (EBL) conferred a dramatic change in red blood cell invasion in mutant rodent malaria parasites Plasmodium yoelii. In the same experiment, we implicated merozoite surface protein 1 (MSP1) and other polymorphic proteins, as the major targets of strain-specific immunity. Using allelic replacement, we provide functional validation of the substitution in the EBL gene controlling the growth rate in the blood stages of the parasites.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmodium yoelii
/
Proteínas de Protozoários
/
Receptores de Superfície Celular
/
Proteína 1 de Superfície de Merozoito
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Malária
/
Antígenos de Protozoários
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
PLoS Pathog
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Japão