Short stretches of rare codons regulate translation of the transcription factor ZEB2 in cancer cells.
Oncogene
; 36(47): 6640-6648, 2017 11 23.
Article
em En
| MEDLINE
| ID: mdl-28783176
ABSTRACT
Two proteins comprising the ZEB family of zinc finger transcription factors, ZEB1 and ZEB2, execute EMT programs in embryonic development and cancer. By studying regulation of their expression, we describe a novel mechanism that limits ZEB2 protein synthesis. A protein motif located at the border of the SMAD-binding domain of ZEB2 protein induces ribosomal pausing and compromises protein synthesis. The function of this protein motif is dependent on stretches of rare codons, Leu(UUA)-Gly(GGU)-Val(GUA). Incorporation of these triplets in the homologous region of ZEB1 does not affect protein translation. Our data suggest that rare codons have a regulatory role only if they are present within appropriate protein structures. We speculate that pools of transfer RNA available for protein translation impact on the configuration of epithelial mesenchymal transition pathways in tumor cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
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Códon
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RNA de Transferência de Glicina
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RNA de Transferência de Leucina
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RNA de Transferência de Valina
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Homeobox 2 de Ligação a E-box com Dedos de Zinco
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Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
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NEOPLASIAS
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido