Molecular Determinants of Temperature Dependence of Protein Volume Change upon Unfolding.
J Phys Chem B
; 121(35): 8300-8310, 2017 09 07.
Article
em En
| MEDLINE
| ID: mdl-28795561
ABSTRACT
Pressure is a well-known environmental stressor that can either stabilize or destabilize proteins. The volumetric change upon protein unfolding determines the effect of pressure on protein stability, where negative volume changes destabilized proteins at high pressures. High temperature often accompanies high pressure, for example, in the ocean depths near hydrothermal vents or near faults, so it is important to study the effect of temperature on the volumetric change upon unfolding. We previously detailed the magnitude and sign of the molecular determinants of volumetric change, allowing us to quantitatively predict the volumetric change upon protein unfolding. Here, we present a comprehensive analysis of the temperature dependence of the volumetric components of proteins, showing that hydration volume is the primary component that defines expansivities of the native and unfolded states and void volume only contributes slightly to the folded state expansivity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Proteínas
/
Desdobramento de Proteína
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Phys Chem B
Assunto da revista:
QUIMICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos