Evolutionarily Conserved Principles Predict 3D Chromatin Organization.
Mol Cell
; 67(5): 837-852.e7, 2017 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-28826674
ABSTRACT
Topologically associating domains (TADs), CTCF loop domains, and A/B compartments have been identified as important structural and functional components of 3D chromatin organization, yet the relationship between these features is not well understood. Using high-resolution Hi-C and HiChIP, we show that Drosophila chromatin is organized into domains we term compartmental domains that correspond precisely with A/B compartments at high resolution. We find that transcriptional state is a major predictor of Hi-C contact maps in several eukaryotes tested, including C. elegans and A. thaliana. Architectural proteins insulate compartmental domains by reducing interaction frequencies between neighboring regions in Drosophila, but CTCF loops do not play a distinct role in this organism. In mammals, compartmental domains exist alongside CTCF loop domains to form topological domains. The results suggest that compartmental domains are responsible for domain structure in all eukaryotes, with CTCF playing an important role in domain formation in mammals.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Cromatina
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Proteínas Cromossômicas não Histona
/
Histonas
/
Proteínas de Drosophila
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Montagem e Desmontagem da Cromatina
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Drosophila melanogaster
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Mol Cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos