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RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancers.
Hoang, Van L T; Tom, Lisa N; Quek, Xiu-Cheng; Tan, Jean-Marie; Payne, Elizabeth J; Lin, Lynlee L; Sinnya, Sudipta; Raphael, Anthony P; Lambie, Duncan; Frazer, Ian H; Dinger, Marcel E; Soyer, H Peter; Prow, Tarl W.
Afiliação
  • Hoang VLT; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Tom LN; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Quek XC; Garvan Institute of Medical Research, Sydney, New South Wales, Australia.
  • Tan JM; St Vincent's Clinical School, University of New South Wales, Sydney, New South Wales, Australia.
  • Payne EJ; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Lin LL; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Sinnya S; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Raphael AP; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Lambie D; Dermatology Research Centre, Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Frazer IH; Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.
  • Dinger ME; Department of Anatomical Pathology, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
  • Soyer HP; Diamantina Institute, Translational Research Institute, Princess Alexandra Hospital, The University of Queensland, Brisbane, Queensland, Australia.
  • Prow TW; Garvan Institute of Medical Research, Sydney, New South Wales, Australia.
PeerJ ; 5: e3631, 2017.
Article em En | MEDLINE | ID: mdl-28852586
ABSTRACT
Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, we have utilised next generation RNA sequencing (RNA-seq) to analyse the transcriptome of a panel of non-melanoma skin cancer lesions, identifying genes that are consistently expressed across all samples. Genes encoding ribosomal proteins were amongst the most stable in this dataset. Validation of this RNA-seq data was examined using qPCR to confirm the suitability of a set of highly stable genes for use as qPCR RGs. These genes will provide a valuable resource for the normalisation of qPCR data for the analysis of non-melanoma skin cancer.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália