dCas9: A Versatile Tool for Epigenome Editing.
Curr Issues Mol Biol
; 26: 15-32, 2018.
Article
em En
| MEDLINE
| ID: mdl-28879853
ABSTRACT
The epigenome is a heritable layer of information not encoded in the DNA sequence of the genome, but in chemical modifications of DNA or histones. These chemical modifications, together with transcription factors, operate as spatiotemporal regulators of genome activity. Dissecting epigenome function requires controlled site-specific alteration of epigenetic information. Such control can be obtained using designed DNA-binding platforms associated with effector domains to function as targeted transcription factors or epigenetic modifiers. Here, we review the use of dCas9 as a novel and versatile tool for fundamental studies on epigenetic landscapes, chromatin structure and transcription regulation, and the potential of this approach in basic research in these fields.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
DNA
/
Genoma Humano
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RNA Guia de Cinetoplastídeos
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Endonucleases
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Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
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Edição de Genes
Limite:
Humans
Idioma:
En
Revista:
Curr Issues Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Holanda