Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae.

Temer, Beatriz; Dos Santos, Leandro Vieira; Negri, Victor Augusti; Galhardo, Juliana Pimentel; Magalhães, Pedro Henrique Mello; José, Juliana; Marschalk, Cidnei; Corrêa, Thamy Lívia Ribeiro; Carazzolle, Marcelo Falsarella; Pereira, Gonçalo Amarante Guimarães

Temer, Beatriz; Dos Santos, Leandro Vieira; Negri, Victor Augusti; Galhardo, Juliana Pimentel; Magalhães, Pedro Henrique Mello; José, Juliana; Marschalk, Cidnei; Corrêa, Thamy Lívia Ribeiro; Carazzolle, Marcelo Falsarella; Pereira, Gonçalo Amarante Guimarães.

Afiliação

Temer B; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Dos Santos LV; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Negri VA; CTBE - Brazilian Bioethanol Science and Technology Laboratory, Campinas, SP, Brazil.
Galhardo JP; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Magalhães PHM; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
José J; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Marschalk C; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Corrêa TLR; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Carazzolle MF; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
Pereira GAG; Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.

BMC Biotechnol ; 17(1): 71, 2017 09 09.

Article em En | MEDLINE | ID: mdl-28888227

Assuntos

Aldose-Cetose Isomerases/metabolismo; Chaperonina 60/genética; Proteínas de Escherichia coli/genética; Proteínas de Choque Térmico/genética; Engenharia de Proteínas/métodos; Saccharomyces cerevisiae/genética; Aldose-Cetose Isomerases/química; Aldose-Cetose Isomerases/genética; Chaperonina 60/metabolismo; Proteínas de Escherichia coli/metabolismo; Etanol/metabolismo; Proteínas de Choque Térmico/metabolismo; Modelos Moleculares; Propionibacterium/enzimologia; Conformação Proteica; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Saccharomyces cerevisiae/metabolismo; Xilose/metabolismo

Palavras-chave

Ethanol production; GroEL-GroES chaperonins; Saccharomyces cerevisiae; Xylose fermentation; Xylose isomerase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Engenharia de Proteínas / Chaperonina 60 / Aldose-Cetose Isomerases / Proteínas de Escherichia coli / Proteínas de Choque Térmico Tipo de estudo: Prognostic_studies Idioma: En Revista: BMC Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil

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