[Determination of stachydrine hydrochloride and leonurine hydrochloride in Yimucao preparations by HPLC-MS].
Zhongguo Zhong Yao Za Zhi
; 41(10): 1876-1879, 2016 May.
Article
em Zh
| MEDLINE
| ID: mdl-28895336
ABSTRACT
To establish the quantitative method of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba. The contents of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba were determined by HPLC-MS. The chromatographic column was Waters XBridge Amide(4.6 mm×250 mm,5 µm). The mobile phase was acetonitrile-0.1% formic acid in gradient mode,at the flow rate of 1.0 mL⢠min⻹,with the split ratio of 1â¶4. MS conditions for the ESI ion source,positive ion mode,selective ion scan(SIM) of stachydrine hydrochloride(m/z 144.0) and leonurine hydrochloride(m/z 312.0) was measured. The linear ranges of stachydrine hydrochloride was 0.562 8-281.4 µgâ¢L-1ï¼r=0.999 8ï¼. The linear ranges of leonurine hydrochloride was 0.521 2-260.6 µgâ¢L-1ï¼r=0.999 8ï¼. The method is accurate,simple,and reliable,and can be used to determine the contents of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prolina
/
Medicamentos de Ervas Chinesas
/
Leonurus
/
Ácido Gálico
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China